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PCR-TRAP Cloning Vector ligated with a 450 bp PCR amplified LacZ gene was transformed into E. coli and plated on a tetracycline plate. Blue colonies are
Minocycline, a semisynthetic tetracycline derivative, protects brain . Cells were cultured on poly-l-lysine-coated 96-well plates (1 × 105 cells
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The majority of positive results on plates seeded with B. subtilis, 77 of 89 samples tested, contained tetracycline antibiotics. One sample also contained
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Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 ug/ml) containing agar plates or
Electrocompetent E. coli cells with an aliquot of the in vitro insertion reaction, insertion clones were selected by growth on tetracycline plates. Greater than > 106
Cells were grown overnight at 28°C in tetracycline-containing LBS liquid medium spotted onto an LBS plate containing 0.3% glycerol (vol/vol) and tetracycline.
The Abraxis Tetracycline Plate Kit is a competitive ELISA for the quantitative analysis of Tetracycline in. Honey. ASSAY PRINCIPLES. The Abraxis Tetracycline
Only one of the colonies recovered from the 25-µg/ml tetracycline plates was able to grow consistently in high tetracycline concentrations.
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The present study documents the instability of minocycline when stored under refligeration in agar plates. Doxycyc[ine and tetracycline also lost activity but
Streak XLI-Blue MRF' from frozen stock on an LB-tetracycline plate. 2. Incubate plate overnight at 37°C. Streak SOLR strain from frozen stock on an
An improved medium for the direct, positive selection of tetracycline-sensitive clones from a population Tce plates, rich broth plates containing 20 ,ug of tetra-
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However, by the late afternoon, colonies were visible on all of the plates except for the tetracycline plates. We continued working on the wiki and researching
LB + tetracycline plates (10 ug/ml)--1 week old or less; red stripe. LB+ampicillin ( 100 ug/ml) + tetracycline plates (10 ug/ml)--1 week old or less; red and green
tetracycline plates were overlaid with an additional 2.5 ml of top agar containing then streaked onto M9 lactose-tetracycline plates to confirm that they were
The direct plating gives positive results but the transformation of the minipreps gives no colonies on tetracycline plates. After some researches
No lawn and no visible signs of bacteria appeared on the plate with tetracycline. An equivalent number of bacteria, chemically treated to make
Agar plates that include the selective marker. For example, if you want to transduce a tetracycline marker you need LB tetracycline plates. 6. “Green plates”
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Abstract—Tetracycline (TC) resistance gene tet(M) is reported to distribute in grown on LB plate contained 20 µg/ml of TC at 42°C for 24 h. E. faecalis
tetracycline was stable at k20 mC for many weeks, and was soluble in LB agar plate medium. Ethylated tetra- cycline induced growth inhibition of XL1-Blue
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LB plates containing 25 Hg/ml tetracycline. Marker rescue. The marker rescue assay, which is a modification of the one used by. Smit,h (1968) to measure
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Unfortunately the next day we did not get any colonies for all the 3 plates. It seems that the Tetracycline plates were not effective. As we were not sure about the
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cally investigated using a modified four-plate test (MFPT). Four culture The identification and quantification of tetracycline residues in the raw meat was per-
cultures were spread on ampicillin agar plates while the rest of the transformation mixture was spread on tetracycline plates. The plates were incubated for
grown on the nutrient agar plates containing 8-16. µg/mL tetracycline, as well as antibiotic free media plates as controls. The resistant colonies were selected for
Plates were incubated at 55 + 1šC for 6 hours. Tetracycline plates: 25 g/L of Standard II Närh-agar lyophilised at pH 6.0 ą 0.1 with 0.5% of a 100 ĩg/kg solution of
plate containing 15 inicrograms/ml tetracycline that has been spread with 5 x IO7 - 2 X IO8 cells of a tetracycline-sensitive homo-immune h lysogen. Plates were
0.3ml of P. aeruginosa in nutrient broth and the plates were incubated for 72 hours at 37 degrees. A powder of tetracycline was purchased from Sigma.
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Heat 600 ml deionized water in 1 liter beaker on hot plate with stirring. Gradually add . Note: Magnesium ions are antagonists of tetracycline. Do not use with
120 and 240 µg/ml of Tetracycline. Sediments were mixed with sterile PBS and made 10 fold dilution and then inoculated on to agar plate. Agar plates were
plated in duplicate with the tetracycline and control medium. The plates were incubated at. 30 C, one set for 48 hr and the other for 72 hr. Counts are shown in
Tetracycline gutta percha, Calcium hydroxide gutta percha and traditional gutta percha cones were aseptically transferred onto each of the inoculated plates
o Colonies which grew on ampicillin but not later on tetracycline plates are the successful recombinants. Amp. Amp. Tet. Tet. Master plate (ampicillin). Replica
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High prevalence of tetracycline resistance in Enterococcus isolates from .. Antimicrobial-free agar plates were included as a control for normal
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Tetracycline does not need to be added to media during phage amplification . 3 . Streak out ER2738 from the supplied glycerol culture onto an LB+Tet plate .
Newmarket Scientific Product Search Results for Tetracycline (TET) The drug of interest has been coated in the plate wells. During the analysis, sample is
showed higher initial numbers of colonies than identical samples grown on tetracycline plates (6). Increases in the concentration of ampicillin and tetracycline in
With a loop, carefully scrape off one colony from either the ampicillin or tetracycline plate and inoculate a tube of L broth and a tube of L broth containing the
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The phenotype of the transductants obtained was tested by tooth-picking colonies to Mueller-Hinton (MH) plates containing tetracycline (1 µg/ml, for full induction
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All of the E. coli should be susceptible to ampicillin and die on ampicillin + tetracycline plates. Unchanged V. harveyi in the experimental group will not tolerate
each of the two 10 ml sub-cultures were plated onto 3 agar plates with 0.5 ug/ml tetracycline (a potentially lethal dose to wild type bacteria) and 3 agar plates
to approximately 55°C. If kept at 4°C tetracycline, chloramphenicol, and streptomycin plates are usually good for several months, but kanamycin plates and
All bacterial plates were sensitive to their target antibiotics, except . tetracyclines (left panel) and the plate sensitive to sulfonamides (right panel) after 20 h of
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Protocol for the preparation of LB liquid media and agar plates with Tetracycline. Tryptone. 10. Yeast Extract. 5. NaCl. 10. Agar (if preparing agar plates). 15. 2
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The tetracyclines (tetracycline, doxycycline, demeclocycline, minocycline, etc.) . Inoculate a 150mm Mueller-Hinton agar plate with the standardized inoculum
In the case of genetic determinants for tetracycline resistance, it has been proposed . This pattern may have been amplified by the use of selective plates for
The zones of inhibition for the tetracycline plate were also tracked for the entire experiment. Figure 5 shows the sensitivity to both Tet and Dox that changed over
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Replication of plates. 1. Prepare target plates by dispensing ~160µl of LB media, 8% glycerol, ampicillin at a concentration of 100 µg/ml, and tetracycline at a
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When chloramphenicol, tetracycline, or metronidazole was added to plates, the amphotericin B and vancomycin were omitted . E . coli DH5
The plates were prepared with commercial grade Kieselguhr, and buffered at pH 7.5. Solutions. of tetracycline hydrochloride containing x0-5o l~g[pl were
This strain grows on LB or 2xYT plates (and is resistant to tetracycline, see below ), and competent cells can be made using standard techniques. Note on
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replication, transposon insertion mutants were selected on LB-tetracycline plates. (15 g/ml). The mutants were screened for the desired phenotype by comparing
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The colonies on the first plate are transferred to identical positions on a new plate containing Tetracycline. Only bacterial colonies containing plasmids will grow.
spiked, undiluted meat. The thick plates were suited for doxycycline and chlortetracycline, but they did not detect oxytetracycline or tetracycline in spiked meat
Each of the 4 plates was divided into four sections, testing 8 different antibiotics: 1 ) Ampicillin 2) Ciprofloxacin 3) Tetracycline 4) Vancomycin 5) Erythromycin 6)
Make a 4.105 dilution of the glycerol stock; Plate 150 µl of the dilution on Q-tray with 200 ml of LB + 5 µg/ml tetracycline + 50 µg/ml rifampicin + 50 µg/ml
strains 26695, J99, SS1, and ATCC43504. Individual bacterial colonies present on tetracycline-containing plates (2 g/ml) were selected, and their MICs of tetra-
Four antibiotic disks were dispensed on each plate. The disks contained 10 µg of ampicillin (top left), 30 µg of tetracycline (top right), 30 µg of
If you use plates that are less than six months old and are not too dry, you should have good results. The only plates to be careful about are tetracycline plates.
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Plates containing 2% yeast extract, 1% peptone, and 2% glucose (w/v) (YPD) supplemented with 120 μg/ml tetracycline where used for conditional knock-
mixture on separate LB-tetracycline agar plates. (Prior to plating the cells, place 100 µl of SOC medium on each. LB-ampicillin or LB-tetracycline plate. Pipet the
To find the plates search for the plate number in the line name field of the The plasmid pSoup carries a gene that confers resistance to tetracycline in bacteria.
Plates containing methicillin, erythromycin, cephalothin, tetracycline, chloramphenicol, kanamycin, streptomycin, polymyxin B, or nalidixic acid
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Allow the autoclaved medium to cool to 55°C. For LB ampicillin plates, add ampicillin to a final concentration of 100µg/ml. For LB tetracycline
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LB agar plates containing tetracycl ine (10µg/mL). These plates are needed only if a tetracycline-resistant strain of E. coli,such as XL1-Blue is used to propagate
ing strains which is known as the STAR five-plate method (22). It is used for detection of antibiotics from the macrolide, aminoglicoside, tetracycline and
plates. Tetracycline plates are 10 ug/ml, made as follows: Allow the autoclaved LB media to cool to ca. 48° C. Add 10.0 mLs of the tetracycline stock per liter of
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Note: prepare enough plates (with extra ones to replace contaminated plates). II. Other materials: 5. Antibiotics (carbenicillin, tetracycline). 6.
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The shelf life is 12 months when the kit is properly stored. Kit Contents. Amount. Storage. Tetracycline-coated Plate. 1 x 96-well Plate (8 wells x 12 strips) 2-8°C
Streak XL1-blue MRF' cells on LB/tetracycline (12.5µg/ml) plates and grow overnight at 37°C. In a sterile 50ml tube, start a culture from a single colony and grow
C. Cut pBR322 with Bam HI, mix with gene X, ligate, infect E. coli, select for ampicillin resistance and failure of replica colonies to grow on tetracycline plates.
Tetracycline stock (Tet): Stock of 10 mg/ml in 50 % ethanol + sddwater. 1 g Tetracycline 50 ml 100% ethanol
Store plates in the dark at 4 °C. Magnesium ions are antagonists of tetracycline, therefore avoid using media, such as LB that contain magnesium salts.
broth and incubated for 1h at 37 °C prior to plating on PPLO plates (Difco, Detroit, MI) con- taining 2 μg/ml tetracycline. Plates were incubated at 37 °C in a moist
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This medium was supplemented with 10 µg/ml tetracycline to isolate tetracycline resistant (Tetr) enterococci. From the KF agar plates, 100 typical Tetr
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Absence of contamination by plating on five different LB plates containing either 100. µg/ml ampicillin, 50 µg/ml kanamycin, 15 µg/ml tetracycline, 20 µg/ml
Allow the autoclaved medium to cool to 55°C. For LB ampicillin plates, add ampicillin to a final concentration of 100µg/ml. For LB tetracycline plates, add
Streak the XL1-Blue MRF' host bacterial glycerol stock onto the LB-tetracycline ( 12.5 µg/ml) agar plate. Incubate the plate overnight at 37°C.
Results 1 - 10 of 156000 In the presence of nrssDNA, the colony count on tetracycline plates was 2 to 6 in . .. www.ncbi.nlm.nih.gov/pmc/articles/PMC1951227
]-for plasmid transformation, grows well on L broth and plates. I[q]ZDM15, Tn10 (tet[r])] -For plating or glycerol stocks, grow in LB with 20 ug/ml of tetracycline.
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1, GSE1150_GSM19263, CEA_SGF:AHU032;-LIF 16h ARN test 4x105 cells were plated in a 10 cm cell culture plate and cultured in the presence of tetracycline
LB-TET AGAR. -Make 1 liter LB agar. -Autoclave. -Cool to 55°C. -Add 12.5 mg of filter-sterilized tetracycline. -Pour plates and store in a dark, cool place.
They were grown in LB broth and then inoculated on LB agar plates containing kanamycin, tetracycline, or ampicillin to find bacteria resistant to
The LB+ampicillin and LB+tetracycline plates were incubated at 37°C for 12 to 16 and 36 to 40 hours, respectively, and colonies were counted manually.
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A new family of tetracycline compounds, without the ring-attached [0219] Powder samples were prepared as flat plate specimens using.
Penicillin (14%) was the drug with the highest rate of occurrence followed by tetracycline (8%) and streptomycin (4%) in samples positive on a single plate.
Schematic close-ups illustrate cellular growth of the sensitive and resistant strains across the plate. C, Tetracycline-resistant (CFP-labeled strain t17cl, shown in
He noticed that a patch of the mold Penicillium notatum had grown on a plate and tetracycline, and the age of antibiotic chemotherapy came into full being.
E4 coli cells are transformed, then gmwn on agar plates containing tetracycline to select for those that have taken up plasmid. transformation. ofE. coli cells. Host
The recombinant plasmid has an intact tetracycline resistance gene, so the bacteria can be selected by growth on plates containing tetracycline
If a colony grew on the TCS ampicillin, tetracycline plate then that colony is resistant to ampicillin because the bacteria in that colony contain the pET11a plasmid
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For selective plates, 10 [micro]L of slurry was plated. The concentration of tetracycline selected for the [M.sup.T]plates was set below the Clinical and Laboratory
The thick plates were suited for doxycycline and chlortetracycline, but they did not detect oxytetracycline or tetracycline in spiked meat fluid.
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These plates were incubated at 37& 176;C for 48 h in air supplemented with 5% CO2 tetracycline overnight, providing selection against the obligate anaerobic
Colonies which grow on the ampicillin plate could be either type 2 or type 3 but those that also do not grow on the tetracycline plate are the transformants that
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Tetracycline must always be wrapped in foil and stored in the dark whether it is in Plates should be stored upside down at 4oC in the dark (foil wrapped) for no
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Prepare LB agar plates containing 50-100 μg/ml ampicillin (no tetracycline). • Transform 1 μl (10 pg) of pUC19 into 50 μl of competent cells according to the
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No growth on tetracycline plates (15 µg/mL). Confirms absence of plasmid- encoded tetracycline resistance genes. Streptomycin Plates. Growth
Growth inhibition zones on each plate were inspected at 24, 48, and 72 h. Tetracycline disc and TGP cones inhibited all the tested bacterial
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This means that cells sucessfully transformed with the plasmid+insert won't grow on tetracycline plates. Cells with just plain plasmid will. However, tetracycline is
We analyzed 47 colonies recovered from 25-μg/ml tetracycline plates, and most of them had a transient resistance phenotype (not stable),
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Transfer each colony to one well of a 24-well tissue culture plate that contains 1 ml of selection medium containing 250 μM L-histidinol and tetracycline. 19.
I. CULTURE 24 hrs before inoculation, transfer clones onto a fresh agar plate, containting ampicillin and tetracycline. 1. Inoculate clones into 100 ul medium,
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Antibiotics on this plate are: (center) kanamycin (K), (clockwise from upper right) penicillin (P 10), streptomycin (S 10), tetracycline (TE 30), and
Luria-Bertani (LB) Agar Plates Prepoured, 100x15 mm plates with Luria-Bertani ( LB) Agar Contains 50 μg/ml Tetracycline pH: 7.0 Keep away from continuous
SEC plate showed that 74% of all strains agreed within ± 1 log2 dilution . a one to five log2 dilution increased in MICs of E. coli to tetracycline
Tetracycline sensitive variants, such as DT-lIC1, repre- sented colonies that grew on a tetracycline-free. AB3 agar plate but failed to grow on an AB3 agar plate
-Add 20 g Bacto-agar. -Autoclave. LB-TET AGAR. -Make 1 liter LB agar. - Autoclave. -Cool to 55°C. -Add 12.5 mg of filter-sterilized tetracycline. -Pour plates and
Plate #2 antibiotics were placed as follows: vancomycin--place 1; tetracycline-- place 2; ciprofloxacin--place3; ampicillin--place 4. It is important to stress that the
Europium(III)-tetracycline complex adsorbed on the surface of C18 TLC plates generated the most intensive luminescence when measured at lambda ex = 388
If a fungal infection appears on a plate and it seems difficult to pick a clean Chlamy Tetracycline, Cefotaxime, Trimethoprim, erythromycin, ceftixozyme are fine.
Tetracyline-sensitive colonies can be identified by their inability to grow on the tetracycline plates, rescued from the master plate, and analyzed further.
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Product Description REAGEN™ Tetracycline (TET) ELISA Test Kit is a Incubations of assay plates should be timed as precisely as possible.
It is low in sulphonamide, trimethoprim, and tetracycline inhibitors. This corresponds to 60 to 70 ml of medium for plates with diameters of 150 mm and 25 to 30
Spread 0.2 ml from the no-phage control and no-cell control tubes onto separate LB plus tetracycline plates. Spread 0.2 ml from tubes C, D, and E onto separate
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To this end we report the development of a stable tetracycline-dependent expression 1 ml was then transferred to each well of a 24-well plate and the cells
Replication Of Plates. 1. Prepare target plates by dispensing ~160 μl of LB media , 8% glycerol, ampicillin at a concentration of 100 μg/ ml, and tetracycline at a
19959)10%] tetM CAT Prepared as recipient cells Incubation w/ fusion buffer Plated on SP4 agar plates w/ tetracycline & chloramphenicol Only
Bacterial colonies growing on plates containing ampicilin are not replicated onto plats containing tetracycline. Bacteria that have taken up recombinant plasmids
units (CFU) were counted on plates of the dilution providing 10-300 colonies. All morphotypes of bacteria resistant to 10 //g/ml of tetracycline, all black-
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Bunsen burner to eliminate bubbles. Let the agar harden overnight. Store at 4˚C for <1 month. Tetracycline is light-sensitive; LB/tetracycline plates should be
Yeast plates + media POTASSIUM PHOSPHATE BUFFER (for NGM plates). 1) Make .. Tetracycline stock (Tet): Stock of 10 mg/ml in 50% ethanol + sddwater.
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Transformants which carry plasmids with insert should be unable to grow on the tetracycline plate and can be selected from the identical
terramycin- and tetracycline-containing plates. In a bacterial suspension the bottom layer may tend to have more cells and also the top layer may contain more
The transfectants were plated on M199/10%/hygromycin/tetracycline plates. Four clones were obtained. Total genomic DNA from all the clones was digested
preparation of tetracycline hydrochloride. It was compared in results the International Unit for Tetracycline is defined as the 5 Plate Staphylococcus aureus 8
Colonies with human DNA inserted into the tetracycline gene will not grow on tetracycline plates, but will grow on ampicillin plates. Antibiotic-Resistance Genes
think you had the number of colonies you had on the X-GAL plate? 4. The antibiotics tetracycline and kanamycin are also found on antibiotic resistant plasmids.
Plates containing lower concentrations of EB were devoid of any fluorescence ( not shown). B: The presence of 30 μg of tetracycline promotes formation of a
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MC1061/P3 transformed with a supF containing plasmid, we recommend performing double selection on 10 μg/ml tetracycline and 25 μg/ml ampicillin LB plates.
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Replica plate the cells from the tetracycline plates to ampicillin plates. Return to the tetracycline plates and culture the cells that grew on tetracycline but not
Luria plates containing either ampicillin-only (50 µg/mL), tetracycline-only (15 µg/ mL), tetracycline plates (15 µg/mL) selected for fewer transformants than
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LB Agar Plates with Tetracycline-4. 100mm plates, sterile. Cat. No. L0204.
Solutions and plates are light sensitive. Tetracycline is incompatible with ( chelated by) magnesium ions, and thus unsuitable for use in minimal
Tetracycline-integrated gutta-percha (TGP) cones, tetracycline disc, and conventional gutta-percha cones of the same size were placed on the inoculated plates.
PRECAUTIONS. 1. Each reagent is optimized for use in the Abraxis Tetracycline Plate Kit. Do not substitute reagents from any other manufacturer into the test kit.
LB Agar Plates with Tetracycline-2. 100mm plates,sterile. Cat. No. L0202.
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tetracycline plates supplemented with thiamine and theophylline, and the NAND gate cells were plated on M9-A tetracycline plates supplemented with thiamine.
Tetracycline for transformed Agrobacterium. Nobody A simple protocol is to spread cells onto an LB plate together, at a=20 ratio of 2:1:1
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